시베리아에서 직수입한 최상급 차가 버섯!
오늘 하루동안 보지 않기

논문자료

게시판 상세
제목 차가추출물의 항산화(노화방지) 효과 논문 번역본
작성자 관리자 (ip:)
  • 평점 0점  
  • 작성일 2009-02-11
  • 추천 추천하기
  • 조회수 1741

각종 산소 라디칼들이 생체내의 호흡효소계나 산화환원계에서 생성되어 노화와 각종 질병과 유관한 사실이 밝혀져감에 따라 이들의 소거를 위한 항산화물질에 대한 관심이 높아지고 있는 시점에서, 본 논문에서는 차가버섯의 대표 유효성분인 항산화 성분을 최대로 추출해내기 위한 효과적인 추출법을 시도하고자 실행하였다.


차가버섯 을 80℃에서 8시간 추출한 것을 1차 추출물, 1차 추출 후 그 잔사물에 물을 첨가하여 100℃에서 8시간 추출한 것을 2차 추출물, 2차 추출 후 남은 잔사물에 물을 첨가하여 120℃에서 8시간 추출한 것을 3차 추출물로 하여서, DPPH 자유 라디칼에 대한 전자공여능, ABTS·+ 라디칼 양이온 소거능, 리놀레산 자동산화 저해 효과를 봄으로서 항산화능을 측정하였다.


결과에서 항산화력은 1차 추출물이 가장 낮았고 3차 추출물이 가장 높았고, 3차 추출물은 페놀 화합물과 플라보노이드 함량이 1차나 2차 추출물보다 유의적으로 많았고, 페놀화합물 함량은 라디칼 소거능 및 리놀레산 자동산화 저해효과와 상관관계가 높은 것(r=0.960~0.980, regression analysis)으로 나타났다.


또한 각 온도에서의 추출물을 혼합하여 지방산화 촉진인자인 3종의 활성산소종(KO₂, H₂O₂, ㆍOH)과 금속 이온(Fe^(2+), Cu^(2+)) 존재 하에서 항산화 효과를 TBARS값으로 측정한 결과, 차가추출물이 모두의 경우에서 TBARS값을 낮추었으며 특히 ㆍOH와 Fe^(2+) 이온에 대하여 탁월한 항산화능을 보였다.



이상의 결과로 미루어 보아 자유 라디칼로 인해 야기되는 지방을 비롯한 그 산화물에 대하여 3단계 온도 추출법에 의한 차가버섯 추출물은 매우 유효한 항산화제로서 작용할 수 있을 것으로 사료되어진다.

 

영어 초록

The efficacy of extraction from Inonotus obliquus was examined from the points of antioxidative characteristics and some antioxidative compounds. To enhance the efficient extraction for the effective components from Inonotus obliquus, temperature-stepwise water extraction method was applied. Temperature-stepwise water extracts were prepared for 8 hrs as follows: the first extract at 80℃, the second extract from the residue of the first extract at 100℃, and the third extract from the residue of the second extract at 120℃. Antioxidative activities were determined by electron-donating ability of DPPHㆍ free radical, scavenging ability of ABTSㆍ^+ radical cation, and by inhibiting ability of linoleic acid autoxidation. In results, the first extract showed the least antioxidant capacity, and the third extract showed the highest antioxidant capacity. The third extract also had the greatest amounts of phenolic compounds and flavonoids. Amounts of phenolic compound from each extract were almost proportional to the radical scavenging activities and linoleic acid autoxidation inhibiting ability (r=0.960~0.980, regression analysis). Furthermore, the effect of the pooled extract of all three extractions of Inonotus obliquus on the lipid peroxidation reacted with active oxygen species (KO₂, H₂O₂, ㆍOH) and metals (Fe^(2+), Cu^(2+)) was evaluated by measuring the formation of thiobarbituric acid reactive substances (TBARS). The pooled Inonotus obliquus extracts lowered the amounts of TBARS formed by all of the active oxygen species and metals. Especially, these lowering effects were pronounced in the reaction with ㆍOH and Fe^(2+). These results suggest that the pooled temperature-stepwise extract from Inonotus obliquus could be potential functional materials to reduce the oxidation of lipids and other compounds induced by free radicals.
참고 문헌 정보
⊙ Yoshhiko O. 1989. SOD and active oxygen modulators. NIHON-IGAKUKAN, Tokyo. p 129-278.
⊙ Lee SE. 2001. Antioxidative chracteristics of Chamchwi (Aster scaber Thunb.) and identification of the active compounds. PhD Dissertation. Pusan National University. p 1-30.
⊙ Halliwell B, Gutteridge JMC. 1984. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J 219: 1- 14.
⊙ Maeura Y, Weisburger JH, Williams G. 1984. Dose-dependent reduction of N-2-fluorenylacetamide-induced liver cancer and enhancement of bladder cancer in rats by butylated hydroxytoluene. Cancer Res 44: 1604-1610.
⊙ Branen AS. 1975. Toxcology and biochemistry of butylated hydroxyanisole and butylatedihydroxytoluene. J Am Oil Chem Soc 1: 59-63.
⊙ Jialal I, Grundyl S. 1992. Effects of dietary supplementation with alpha-tocopherol on the oxidative modification of low density lipoprotein. J Lipid Res 33: 899-906.
⊙ Vinson JA, Hontz BA. 1984. Phenol antioxidative index: Comparative antioxidant effectiveness of red and white wines. J Agric Food Chem 43: 401-403.
⊙ Kahlos K. 1994. XII. Inonotus obliquus (Chaga Fungus): In vitro culture and the production of inotodiol, sterols, and other secondary metabolites. Biotechnology in Agriculure and Forestry 26: 179-198.
⊙ Shivrina AN. 1967. Chemical characteristics of compounds extracted from Inonotus obliquus. Chem Abstr 66: 17271z.
⊙ Kier L. 1961. Triterpenes of Poria obliqua. J Pharm Sci 50: 471-474.
⊙ Kahlos K, Hiltunen R. 1983. Identification of some lanostane type triterpenes from Inonotus obliquus. Acta Pharm Fenn 92: 220-224.
⊙ Kahlos T, Zhuangas L, Hitunen R. 1987. Antitumor activity of some compounds and fractions from an n-hexene extract of Inonotus obliquus. Acta Pharm Fenn 96: 33-40.
⊙ Mizno T, Zhuang C, Abe K, Okamoto H, Kito T, Ukai S, Leclerc S, Meijer L. 1999. Antitumor and hypoglycemic activities of polysaccharides from the sclerotia and mycelia of Inonotus obliquus. (Pers.:Fe.) PII (Aphyllophoromycetideae) Int J Med Mushrooms 1: 3
⊙ Ichimura T, Watanave O, Maruyama S. 1998. Inhibition of HIV-1 protease by water-soluble lignin-like substance from an edible mushroom, Fuscoporia obliquq. Biosci Biotechnol Biochem 62: 575-577.
⊙ Saitoh A, Sato C, Niiyama K. 1996. チャガ カバノアナダケ の変異原性 抑制效果に ついて. 道衛硏究報 第46集.
⊙ 星崎 東明 1998. カバノアナダケ (チャガ). 健全社, オれンジ文庫. p 38.
⊙ Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 26: 1199-1204.
⊙ Kim JH, Park JH, Park SD, Choi SY, Seong JH, Moon KD. 2002. Preparation and antioxidant activity of health drink with extract powders from safflower (Carthamus tinctorius L.) seed. Korean J Food Sci Technol 34: 617-624.
⊙ Leong LP, Shui G. 2002. An investigation of antioxidant capacity of fruits in Singapore markets. Food Chem 76: 69-75.
⊙ Osawa T. 1981. A novel type of antioxidant isolated from leaf was of Eucalypyus leaves. Agric Biol Chem 45: 735- 739.
⊙ Choi Y, Him M, Shin J, Park J, Lee J. 2003. The antioxidant activities of the some commercial teas. J Korean Soc Foof Sci Nutr 32: 723-727.
⊙ Moreno MI, Isla MI, Sampietro AR, Vattuone MA. 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71: 109-114.
⊙ Buege JA, Aust SD. 1978. Microsomal lipid peroxidation. Method Enzymol 52: 302-306.
⊙ Cho SY, Han YB, Shin KH. 2001. Screening for antioxidant activity of edible plants. J Korean Soc Food Sci Nutr 30: 133-137.
⊙ Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner AA. 1993. A novel method for measuring antioxidant capacity and its applycation to monitoring the antioxisant status in premature neonates. Clin Sci 24: 407-412.
⊙ Roberta RE, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an im proved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237.
⊙ Wang MF, Shao Y, Li JG, Zhu NQ, Rngarajan M, Lavoie EJ, Ho CT. 1998. Antioxidative phenolic compounds from sage (Salivia officinalis). J Agric Food Chem 46: 4869-4873.
⊙ Kang WW, Kim GY, Park PS, Park MR, Choi SW. 1996. Antioxidative properties of persimmon leaves. Food and Biotechnology 5: 48-53.
⊙ Ka¨hko¨nene MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja RK, Kujala TS, Heinonen M. 1999. Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem 47: 3954-3962.
⊙ Frenkel EN, Huang SW, Kanner J, German JB. 1994. Interfacial phenomena in the evaluation of antioxidants: bulk oils vs emulsion. J Agric Food Chem 42: 1054-1059.
⊙ Banni S, Contini MS, Angioni E, Delana G, Dessi MA, Melis MP, Carta G, Corongiu FP. 1996. A novel approach to study linoleic acid autoxidation: importance of simultaneous detection of the substrate and its derivative oxidation products. Free Radical Resea
⊙ Park YK, Koo MH, Ikegaki M, Contado JL. 1997. Comparison of the flavonoid aglycone contents of Apis mellifera propolis from various regions of Brazil. Arq Biol Technol 40: 97-106.
⊙ Kandaswami C, Middleton EJr. 1994. Free radical scavenging and antioxidant activity of plant flavonoids. In Free radicals in diagnostic medicine. Armstrong D, ed. Plenum Pree, New York and London. p 351-376.
⊙ Ilo M, Moriyama Y, Matsumoto N, Takaki N, Fukumoto M. 1985. Inhibition of xanthine oxidase by flavonoids. Agric Biol Chem 49: 2173-2176.
⊙ Nakagawas T, Yokozawa T. 2002. Direct scavenging of nitric oxide and superoxide by green tea. Food Chem Toxicol 40: 1745-1750.
⊙ Kim SM, Cho YS, Kim EJ, Bae MJ, Han JP, Lee SH, Sung SK. 1998. Effect of hot water extracts of Salivamiltiorrhiza Bge., Prunus persica Stokes, Angelica gigas Nakai and Pinus strobus on lipid oxidation. J Korean Soc Food Sci Nutr 27: 399-405.
첨부파일
비밀번호 수정 및 삭제하려면 비밀번호를 입력하세요.
댓글 수정

비밀번호 :

수정 취소

/ byte

비밀번호 : 확인 취소

고객만족센터

02-512-1180

  1. 메일: befungin@naver.com
  2. 평일: 09:00~19:00 / 토요일: 09:00~15:00

은행계좌 안내

  1. 우리 1005-881-121280
  2. 기업 999-1122-3344
예금주: ㈜야로슬라블코리아